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master cycler nexus gradient thermal cycler  (Eppendorf AG)


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    Structured Review

    Eppendorf AG master cycler nexus gradient thermal cycler
    Master Cycler Nexus Gradient Thermal Cycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 202216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nexus+gradient+master+cycler/Eppendorf/pm41896298-227-11-17
    Average 99 stars, based on 202216 article reviews
    master cycler nexus gradient thermal cycler - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Effect of wild ginseng on the laying performance, egg quality, cytokine expression, ginsenoside concentration, and microflora quantity of laying hens
    Article Snippet: .. Reverse transcription was carried out in an Eppendorf flexid, nexus gradient master cycler, SN:6332kl132036, Germany with annealing (25°C, 10 min), enzyme inactivation (95°C, 5 min). .. Relative tumor necrosis factor alpha (TNF-a) gene was quantified using 5 μL cDNA, 1.75 μL Aqua dest., 2 μL of TaqMan Master Mix and 0.25 μL of both forward and reverse primers, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was employed as the house keeping gene (Bioneer, Daejeon, Korea).

    Amplification:

    Article Title: Serotype distribution and antimicrobial susceptibility patterns of Streptococcus pneumoniae isolates in the post–pneumococcal conjugate vaccine era in Nigeria: a hospital-based cross-sectional study
    Article Snippet: .. Amplification conditions for the PCR were as follows: initial denaturation for 10 min at 94 °C, followed by 35 cycles of denaturation at 94 °C for 15 s, primer annealing at 59 °C for 60 s, and strand extension at 72 °C for 60 s. A final extension was at 72 °C for 5 min on an Eppendorf Nexus Gradient Master cycler (Eppendorf, Germany). .. PCR products were separated on a 2% agarose gel, and DNA bands were visualized with ethidium bromide (New England Biolabs Inc.).

    Article Title: Serotype distribution and antimicrobial susceptibility patterns of Streptococcus pneumoniae isolates in the post-pneumococcal conjugate vaccine era in Nigeria: a hospital-based cross-sectional study.
    Article Snippet: .. Amplification conditions for the PCR were as follows: initial denaturation for 10 min at 94 °C, followed by 35 cycles of denaturation at 94 °C for 15 s, primer annealing at 59 °C for 60 s, and strand extension at 72 °C for 60 s. A final extension was at 72 °C for 5 min on an Eppendorf Nexus Gradient Master cycler (Eppendorf, Germany). .. PCR products were separated on a 2% agarose gel, and DNA bands were visualized with ethidium bromide (New England Biolabs Inc.).

    Article Title: Influence of Food Waste and Graphene Oxide Nanosheets on Monoraphidium sp. VV1 Biomass and Biodiesel Production
    Article Snippet: 1 Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, 625021 Madurai, Tamil Nadu, India 2 Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, 625021 Madurai, Tamil Nadu, India 3 Department of Inorganic Chemistry, School of Chemistry, Madurai Kamaraj University, 625021 Madurai, Tamil Nadu, India 4 Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, 625021 Madurai, Tamil Nadu, India 5 Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), 695019 Thiruvananthapuram, Kerala, India 6 Department of Chemical Engineering, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India 7 Department of Chemistry, K.Ramakrishnan College of Technology (Autonomous), Samayapuram, 621112 Tiruchirapalli, India Abstract From thelast two decades, the microalgal applications in sustainable biodiesel production is gaining attention due to the diminution of fossil fuels and rising fuel prices.. There are different types of microalgae species have been characterized for lipid production under different nutrient starvation and supplementation condition.. Herein, the indigenous microalga Monoraphidium sp. VV1 isolate was used for higher lipid accumulation.

    Polymerase Chain Reaction:

    Article Title: Serotype distribution and antimicrobial susceptibility patterns of Streptococcus pneumoniae isolates in the post–pneumococcal conjugate vaccine era in Nigeria: a hospital-based cross-sectional study
    Article Snippet: .. Amplification conditions for the PCR were as follows: initial denaturation for 10 min at 94 °C, followed by 35 cycles of denaturation at 94 °C for 15 s, primer annealing at 59 °C for 60 s, and strand extension at 72 °C for 60 s. A final extension was at 72 °C for 5 min on an Eppendorf Nexus Gradient Master cycler (Eppendorf, Germany). .. PCR products were separated on a 2% agarose gel, and DNA bands were visualized with ethidium bromide (New England Biolabs Inc.).

    Article Title: Serotype distribution and antimicrobial susceptibility patterns of Streptococcus pneumoniae isolates in the post-pneumococcal conjugate vaccine era in Nigeria: a hospital-based cross-sectional study.
    Article Snippet: .. Amplification conditions for the PCR were as follows: initial denaturation for 10 min at 94 °C, followed by 35 cycles of denaturation at 94 °C for 15 s, primer annealing at 59 °C for 60 s, and strand extension at 72 °C for 60 s. A final extension was at 72 °C for 5 min on an Eppendorf Nexus Gradient Master cycler (Eppendorf, Germany). .. PCR products were separated on a 2% agarose gel, and DNA bands were visualized with ethidium bromide (New England Biolabs Inc.).

    Article Title: Characterization of Pseudomonas mediterranea and Pseudomonas viridiflava strains associated with tomato pith necrosis in the Al Taif region, Saudi Arabia.
    Article Snippet: .. PCR reactions were run on a Nexus Gradient Master Cycler [Eppendorf, Hamburg, DE] using the following profile: initial denaturation at 94 °C for 2 min, followed by 30 cycles of denaturation at 94°C for 2 min, annealing temperature at primer-specific temperatures (Table 1) for 1 min, and extension at 72°C for 1 min. MLST was carried out by concatenating the sequences of the five housekeeping genes (rpoB, rpoD, cts, pfk, and gyrB) into a 3,545 bp alignment for 16 local isolates plus 49 reference sequences AR TIC LE IN PR ES S retrieved from GenBank representing P. mediterranea, P. viridiflava, P. syringae, P. corrugata, and P. cichorii) (Table S2). ..

    Article Title: A cheap two-step cultivation of Phaeodactylum tricornutum for increased TAG production and differential expression of TAG biosynthesis associated genes.
    Article Snippet: A cheap cultivation of microalgae greatly reduces the biodiesel production cost.. Subsequently in this study, citric acid and effluents from sugar and tannery industries were used as the nutritional supplements for the improvement of biomass and TAG production in Phaeodactylum tricornutum using two-step cultivation.. When compared to control (media without supplementation), a considerable increase in biomass and chlorophyll a was obtained with citric acid (CA) and sugar industry effluent (SIE) supplemented media.

    Article Title: Characterization of Pseudomonas mediterranea and Pseudomonas viridiflava strains associated with tomato pith necrosis in the Al Taif region, Saudi Arabia
    Article Snippet: .. PCR reactions were run on a Nexus Gradient Master Cycler [Eppendorf, Hamburg, DE] using the following profile: initial denaturation at 94 °C for 2 min, followed by 30 cycles of denaturation at 94 °C for 2 min, annealing temperature at primer-specific temperatures (Table ) for 1 min, and extension at 72 °C for 1 min. MLST was carried out by concatenating the sequences of the five housekeeping genes ( rpoB, rpoD, cts, pfk , and gyrB ) into a 3545 bp alignment for 16 local isolates plus 49 reference sequences retrieved from GenBank representing P. mediterranea, P. viridiflava, P. syringae, P. corrugata , and P. cichorii ) (Table ). ..

    Standard Deviation:

    Article Title: Discrimination Against Immigrant Latina Mothers Associated With Pro-Inflammatory Gene Expression in Their Preschool-Aged Children.
    Article Snippet: .. M = mean; SD = standard deviation. aNumber and percentage of mothers attributing discrimination to these factors; participants could select more than one attribution. manufacturer’s protocols on an Eppendorf nexus gradient Master cycler. ..

    Control:

    Article Title: A cheap two-step cultivation of Phaeodactylum tricornutum for increased TAG production and differential expression of TAG biosynthesis associated genes.
    Article Snippet: A cheap cultivation of microalgae greatly reduces the biodiesel production cost.. Subsequently in this study, citric acid and effluents from sugar and tannery industries were used as the nutritional supplements for the improvement of biomass and TAG production in Phaeodactylum tricornutum using two-step cultivation.. When compared to control (media without supplementation), a considerable increase in biomass and chlorophyll a was obtained with citric acid (CA) and sugar industry effluent (SIE) supplemented media.



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